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BIOTECHNOLOGY

Development of inovative Biotechnology by obtaining the nanomaterial from Monascus Sp. with potenitial application in therapy (MONALISA)
Development of inovative Biotechnology by obtaining the nanomaterial from Monascus Sp. with potenitial application in therapy
 





MONALISA    

GENERAL INFORMATIONS

  • DEVELOPEMENT OF INOVATIVE BIOTECHNOLOGY BY OBTAINING THE NANOMATERAL FROM MONSACUS SP. WITH POTENTIAL APPLICATION IN THERAPY
  • Contract: 62-070/2008
  • Acronym: MONALISA

PROJECT ABSTRACT

Certain microorganism from Monascus genus, are used traditionally in Asia, as preserving of different meat and fish products. The most used product is called red rice and is obtained by rice fermentation in presence of different Monascus strains. This microorganism produced as metabolites 6 different compounds: rubropunctamin, monascorubramin, rubropunctatin, monascorubin, monascin and ankaflavin. Studies developed on cell lines or on Petri plates reveal that these Monascus metabolites can have some application in medicine due to their antioxidant, healing scar, bacteriostatic and fungistatic effect.

The aims of the project were the following:

  1. obtaining of the modified Monascus strains with high biosynthesis capacities using the parental Monascus species;
  2. getting nanopreparate with Monascus metabolites, their physico-chemical characterization;
  3. characterization of biological activity for bioproducts based on Monascus metabolites, in terms of healing skin lesion effects, antioxidant effects and immunomodulatory properties.


OBJECTIVES

  • obtaining the new pharmaceutical products using clean technologies (biosynthesis which no waste);
  • scientific demonstration and development of technology in order to consolidate the biologic diversities, with obtaining in fact of some high producing microorganism beginning to the parental Monascus sp.. microorganism;
  • optimizing the method to prevent the illness, due to immunity improvement and medical therapy development in the dermatological and immunological field respectively;
  • development the biotechnology of bioactive compounds synthesis, with pharmacology application, with impact to the life quality (due to existing immunity rise) and technological development in the bioengineering and biotechnology fields;
  • development of new materials (metabolites products by modified Monascus microorganism), pharmaceutical products and biotechnological process with high added value
  • rise the competitively and development of organization culture in the education, research and medicine fields


TEAM

COORDINATOR
National Research Institute for Chemistry and Petrochemistry Bucharest-Department of Biotechnology
Partner 1
University POLITEHNICA of Bucharest-Department of Bioengineering and Biotechnology
Partner 2
National Research Institute for Microbiology and Immunology I.C.Cantacuzino - Department of Immunology
Partner 3
University of Medicine and Pharmacy Carol Davila Bucharest Department of Pharmacology


RESULTS

BOOKS:

  1. N. Radu, A. A. Chirvase, 2011. Biotechnology and application of Monascus bio products. Ed. by Printech, ISBN 978-606-521-706-5, Bucharest, Romania.

PAPERS:

  1. N. Radu, l. Rau, C. Corobea, et all., 2012. Properties of Some Biomaterials Obtained by Submerged Biosynthesis, Nonlinear Optic Quantum Optic, (Copernicus) .
  2. N. Radu.; Salageanu, A.; Ferdes; Rau, I., 2012. Cytotoxicity Study Regarding Some Products Derived from Monascus sp., Mol. Cryst. Liq. Cryst., ISSN 1542-1406, vol.555, pp.189-194 (ISI Thomson, Scopus).
  3. N. Radu,; Doncea S.; Ferdes M.; Salageanu A. Rau, I., (2012), Biostimulatory Properties of Monascus sp. Bioproducts. Mol. Cryst. Liq. Cryst. ISSN 1542-1406, vol.555, pp.195-201, ((ISI Thomson, Scopus).
  4. N Radu,; I. Ghita; Caloian, F, I. Rau, 2010. Biopigments, Obtaining and Properties. Mol. Cryst. Liq. Cryst., ISSN 1542-1406 Volume: 523, p. 1-10, (ISI Thomson, Scopus).
  5. Ferdes, M.; Ungureanu, C., 2011. Study of Relationship Between Enzymes Production, Growth Rate and Pigmentogenesis for Five Mutant Strains of Monascus Ruber. REVISTA DE CHIMIE, 62 (1), pp. 75-81. 
  6. C. Ungureanu; M Ferdes, 2010. Antibacterial and antifungal activity of red rice obtained from Monascus purpureus, Chemical Engineering Transactions, 20, pp. 223-228 (Scopus. ISI Thomson).
  7. C.Ungureanu, M.Ferdes, A.Chirvase, N.Radu, 2009. Study of relationship concerning the pigment production and growth rate for five mutant strains of Monascus sp. Chemical Engineering Transaction , ISSN 197-9791, vol. 17, p. 1149-1154 (Scopus, ISI Thomson).
  8. N. Radu, M. Ferdes, O. Coman, I. Ghita Cristescu, C. Ungureanu, 2009. Influence the different submerged media by the production of yellow and red Monsacus sp. pigment ISSN 1222-5339, Scientific Paper, USAMV Bucharest, Series A, vol. LII, (CABI).
  9. M.Ferdes, C.Ungureanu, N.Radu, A. Chirvase, 2009. Antimicrobial effect of Monascus sp red rice against some bacterial and fungal strains. Chemical Engineering Transaction ISSN 197-9791, vol. 17, p. 1089-1094. (Scopus, ISI Thomson).

INTERNATIONAL/NATIONAL COMMUNICATION:

  1. N. Radu, I. G. Cristescu, 2012. Antioxidant properties of traditional bio product based on Monascus sp. (P181), (Abstract in Proceeding Indexed lSI impact, factor 1,5), 13th Congress of the International Society for Ethnopharmacology in collaboration with the Society for Medicinal Plant and Natural Product Research, September 2 - 6, Graz, Austria
  2. N. Radu, C. Corobea, I. Rau, 2012. Processing and characterisation of nanobiosystem obtained from microbial soluble metabolite (8464-39), SPIE NanoScience and Engineering, Conference 8464, 12 - 16 August, San Diego, California, USA
  3. N. Radu, C. Corobea, I. Rau, 2012. Nanobiosystem properties obtained from fungal insoluble metabolite (8464-38), SPIE NanoScience and Engineering, Conference 8464, 12 - 16 August, San Diego, California, USA.
  4. N.Radu, M. Ferdes, C.Corobea, D.Donescu, I.Rau, 2011. Synthesized nanomaterials based on extracellular soluble fungal poliketide, (8103-26), SPIE NanoScience and Engineering, Conference 8103, 23-25 August ,San Diego, California, USA
  5. N.Radu, M. Ferdes, C.Corobea, D.Donescu, I.Rau, 2011. Characterisation of some nanomaterials based on insoluble fungal poliketide, (8103-27), SPIE NanoScience and Engineering, Conference 8103, 23-25 August, San Diego, California, USA
  6. M. Ferdes, C.Ungureanu, N. Radu, A.A. Chirvase, 2009. Antimicrobial effect of Monascus purpureus red rice against some bacterial and fungal strains New Biotechnology, Volume 25, Supplement 1, September, Page S194, ISSN: 18716784 (Abstract Indexed lSI, impact factor 2), International Conference of Biotechnology, Italy, Barcelona.
  7. N. Radu, C. Corobea, M. Ferdes, O. Coman, I.G. Cristescu, C. Ungureanu, A. A. Chirvase, 2009. Nano biomaterial obtaining by innovative biotechnology and their therapeutic application, International Symposium on Chemistry for sustainable development priorities PRIORICHEM, Abstract, p. 20, ISBN 978-973-0-06996-9, 29-30 October, Sinaia, Romania
  8. N. Radu, Cosmin Corobea, Sanda Doncea, et all. 2009, Characterization of microbial pigments produced by microorganisms Ascomyces class, potential raw material for obtaining nanomaterials with applications in therapy. "8th edition of Nanoscience and Nanotechnology of the National Seminar, 27 April.Bucharest, Romania


PROJECT PROGRAM

Year

TASKS

Organi-sation

Term

Total

2008

Task1

Studies regarding the optimization of biosynthesis. Elaboration the growing models and biosynthesis potential. Selection methods of testing of biological activity


20.02.2009

110 895


1.1. Study regarding the best biosynthesis methods of bioactive compounds in the solid state

P1


20.02.2009

50.000


1.2. Experimental models of ‘’in vitro’’ test regarding biological effect of biomaterials obtained from high productive Monascus strains.

P3

20.02.2009

60.895

2009

Task 2

Elaboration the experimental models and method validation used at improved of biosynthesis potential.


10.12.2009

61 206


2.1 Studies regarding the optimising biosynthesis of bioactive compounds in submerged culture

CO

10.12.2009

61 206

2010

Task 3

Experimentation and verification of biological activities of nanomaterials obtained from high productive strains. Results dissemination.


15.11.2010

261 981


3.1. Experimental models for sterilization

P1

15.10.2010

20 000


3.2. Experimental models for cytotoxicity tests ''in vitro'' . Experimental models ''in vitro'' for testing of immunomodulatory and antioxidant properties

P2

15.10.2010

100 000


3.3.Experiments ‘’in vivo’’ regarding scar healing properties of the Monascus biomaterials

P3

15.10.2010

20 000


3.4. Establishment the optimal parameters of biosynthesis in submerged culture. Obtaining the nano biomaterials from parental strain. Physico-chemical characterization of nano biomaterials obtained from parental strains. Results dissemination

CO

20.10.2010

121 981

2011

Task 4

Biological activities validation regarding nano bio preparations obtained from highly productive strains. Dissemination of results.



12.08.2011

361 215.07


4.1. Experimentation model for obtaining highly productive strains. Setting optimal parameters for obtaining highly productive strains

P1

30.07.2011

89 000


4.2. Development of experimental models for in vitro testing of selected bioproducts regarding tissues regeneration

P2

20.07.2011

40 000


4.3.Validation of bio products obtained regarding tissue regeneration. Experiments performed ''in vivo'' with nano products with regard to healing scar effect.

P3

30.07.2011

57 500


4.4. Realization of nano products derived from high productive strains.

Physico-chemical characterization of nano products obtained.

Editing and publishing of book entitled: Development and application of biotechnology to obtain bio product of Monascus sp.

Publish results in revue indexed and quoted ISI

CO

30.07.2011

174 715.07

TOTAL



795 297.07





FINANCIAL RESOURCES

Institution/Partners

2008

(lei)

2009

(lei)

2010 (lei)

(engagement credit )

2011

(lei)

Total

(lei)


B

C

B

C

B

C

B

C

B

C

COORDINATOR

National Institute for Research & Development in Chemistry and Petrochemistry Bucharest

0

0.0

61 206

(ENGAGEMENT CREDIT)

0.0

121981

0.0

14715.07

0.0

357902.07

0.0

PARTNER 1

University POLITEHNICA Bucharest –Department of Biotechnology and Bioengineering

0

0.0

50 000

(BUDGET

STATE)

0.0

20000

0.0

89000

0.0

159000

0.0

PARTNER 2

National Institute for Research & Development in Microbiology and Immunology

INCDMI – CANTACUZINO

0

0.0

0.0

0.0

100000

0.0

40000

0.0

140000

0.0

PARTNER 3

University of medicine and Pharmacy Carol Davila Bucharest- Department of Pharmacology

0

0.0

60.895

(BUDGET

STATE)

0.0

20000

0.0

57500

0.0

138395

0.0

TOTAL

0

0.0

172 101

0.0

261981

0.0

361215.07

0.0

795297.07

0.0


B - BUDGET STATE
C - OTHER FINANCIAL SOURCE




CONTACT

    PROJECT MANAGER
    Dr. Sc. Nicoleta Radu
    I.N.C.D.C.P. ICECHIM Bucuresti
    202 Splaiul Independentei Street, District 6, Bucharest
    E-mail: nicolbiotec@yahoo.com
    tel. 0730.728.694; 021-316.30.72
    fax: 021-312.34.93


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