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BIOTECHNOLOGY Dept.


Inovative systems for fast detetction of contaminants in drinks and foodstuffs (TOX - EVAL)
BIOTECHNOLOGIES
FOR
ENVIRONMENTAL
REMEDIATION
AND PROTECTION
 

















TOX-EVAL    

PROJECT PRESENTATION
  • PROJECT TYPE: Module I CEEX "Excellence in Research" Program
  • FINANCING: National Authority for Scientific Research
  • CONTRACT No: CEEX BIOTECH nr. 68/2006
  • DEVELOPING PERIOD: 1.08.2006 - 15.09.2008
  • The goal of this proposal is to create a complex research team by putting in common, in a synergic way, of entire acquired expertise, in the fields of analytical chemistry, enzymology, electrochemistry, organic chemistry and biotechnology, of the members from four Romanian prestigious institutes: National Institute for Research-Development in Chemistry and Petrochemistry - ICECHIM Bucharest (P1- CO); University of Bucharest through ‘Research Center for Automated Methods of Analysis’ (P2), Chemistry-Physics Institute (P3) and Polytechnic University of Bucharest (P4). As foreign partners, two European institution will collaborate to this project and these are: Universita degli Studi di Roma ’Tor Vergata’ through Dipartimento di Scienze e Tecnologie Chimiche, BioElectroanalytical Tor Vergata (P5) from Roma, Italy and Abo Akademi University, Process Chemistry Centre (P6) from Turku-Abo, Finland. They will participate to the project as non-financed partners.
  • The project main objective consists in the development of new and rapid analytical systems for semi- and quantitative determination of food contaminants, such as heavy metals, nitrites, nitrates, pesticides, mycotoxins and antibiotics. The developed analytical systems will be based on electrochemical and optical sensors, as well as on their integration in flow analysis systems if, in this manner, their performances could be substantially improved.
  • A subsidiary objective is represented by the increase of both, the research activities visibility in Romania in the approached domain, and the constituted partnership institutions involvement in international projects, especially in the next FP7 programs.
  • The scientific managers of the four Romanian partner institutions are specialists at the beginning of their scientific carrier maturity, each of them coming back in Romania after PhD and / or postdoctoral periods of training in European prestigious Universities. They have an appreciable experience in the collaboration / management of national and international research projects, working together in several previous programs. Through the formed consortium in which are also involved the two foreign universities, from Italy and Finland, the transfer and the dissemination of acquired knowledge of the scientific managers to the young researchers brought in the project interest will be achieved.
  • The proposed innovative systems can be used for the entire procedural line of foods, starting with the raw material verifying and ending with the final product. Thus, the project addresses to the programme objectives and the thematic field through "Fork to farm": Food, health and well being through improved quality and safety, both chemical and microbiological, of food, beverage and feed; as well as through the evaluation of environmental impacts on food chains.

PARTNERS
  • CO&P1 - National Research and Development Institute for Chemistry and Petrochemistry - ICECHIM
    Project Manager: Dr. Biochim. Mihaela Badea; m_badea@yahoo.it
  • P2 - Universitatea din Bucuresti, Centrul de Cercetari Metode Automate de Analiza
    P2 Manager: Conf. Dr. Mihaela Carmen Cheregi ; m_cheregi@yahoo.com
  • P3 - Institutul de Chimie Fizica ILIE MURGULESCU, Laboratorul de electrochimie si coroziune
    P3 Manager: Dr. Chim. Cecilia Lete; celete2002@yahoo.com; clete@chimfiz.icf.ro
  • P4 - Universitatea Politehnica din Bucuresti, Facultatea de Chimie Aplicata si Stiinta Materialelor, Catedra de Chimie Analitica
    P4 Manager: Lector dr. Stelian Lupu; stelianl@yahoo.com

OBJECTIVES
  • Studies of obtaining and optimization of analysis systems for heavy metals determination using different strategies depending on foodstuff analyzed nature:
    • Experimentation, optimization and characterization of various manifolds of flow systems with chemiluminometric / electrochemical detection either based on direct determination of heavy metals (Hg, Cu, Mn, Cr, Ni) or on enzymatic inhibition reaction
    • Experimentation and optimization of obtaining procedures of chemical modified electrodes for mercury determination
  • Studies of obtaining and optimization of analysis systems for organic food contaminants determination
    • Studies to obtain modified electrodes with organic and inorganic redox mediators
    • Experimental models to apply the modified electrodes for organic contaminants determination
    • Assembling and characterization of flow systems for antibiotics and pesticides determination
  • Assembling of sensors for ammonia determination in drinkable, spring, well, etc. waters
    • Obtaining and characterization of modified electrodes by electrodepozition of metallic films on carbon paste, glassy carbon or Platinum electrodes
    • Studies concerning the optimization of modified amperometric systems based on copper for ammonia determination
  • Development and optimization of an analysis system for nitrites and nitrated determination
    • Obtaining and characterization of modified electrodes by polymeric films electrodeposition on Platinum electrodes
    • Assembling and optimization of a flow analysis system for the simultaneous determination of nitrites and nitrates in waters
    • Optimization of a system for nitrites determination in meat samples
  • Transfer of expertise regarding the immunosensors technology and immunoassay techniques for mycotoxins determination
    • Studies of immunosensors assembling methods
    • Experimental models of flow immunoassay systems for mycotoxins determination
  • Analysis of (bio)sensors and developed systems analytical performances. Techniques of operational stability improvement according to the product and foodstuff requirements
    • Analytical performances characterization of proposed analytical systems in real working conditions. Studies of potential interferent effects and of their reduction / elimination
    • Testing of assembled systems for contaminants of interest in various foodstuff and matrix effect study
    • Testing and improvement of the operational stability of proposed systems in real working conditions
  • Dissemination of results research activities carried on
    • Participation to national and international scientific events in the project field
    • Publishing of scientific papers in prestigious journals
    • Publishing of a textbook preliminary entitled: "Progresses achieved in food contaminants determination"
  • Participation to international partnershis projects proposals, especially in FP7
    • Meetings for partnership foundation
    • Proposals ellaboration and submission
RESULTS
  • I. Developing of nine original or innovative methods of analysis based on electrochemical sensors / flow injection systems for toxic compounds present in food:
    • 2 methods for mercury
    • 1 method for copper
    • 1 method for total content of heavy metals
    • 1 method for antibiotics
    • 1 method for ammonia
    • 1 method for nitrites
    • 1 method for pesticides
    • 1 method for phenolic toxic compounds
  • II. Human resources development
    • Finalizing of two Ph.D. thesis (Dr. Madalina Bucur and Dr. Cristina Rizea - P2-UB)
    • Two Ph.D. thesis in course of concluding (Drd. Mariana Marin - P3-ICF and Drd. Aleaxandru Chivulescu - P2-UB)
    • Finalizing of a M.Sc. thesis (biochem. Emilia Ocnaru - P1- CO-ICECHIM)
    • Two young researchers employment at P1-CO-ICECHIM
    • Specialization in the field of screening methods for food quality control of the young researchers involved in project
    • Four research stages to University of Rome “Tor Vergata”, Italy
  • III. Development of the research facilities
    • P1-CO - ICECHIM: spectrofluorimeter, portable potentiostat/galvanostat, rotating disk electrode, flow analysis modules
    • P2 - UB: multimeter; fluorescence spectrometer; modules for potentiostat / galvanostat; flow analysis modules
    • P3 - ICF: potentiostat/galvanostat PGSTAT 12
    • P4 - UPB: potentiostat/galvanostat Autolab
  • IV. Increasing of the research results visibility
    • 3 papers in ISI journals
    • 2 papers submitted for publication
    • 8 works presented to national conferences
    • 13 papers presented in frame of international conferences
  • V. New partnerships and reintegration of young researchers from abroad
    • one project PNCDI II - Program Partnerships financed since 2007 (61-030)
    • two proposals PNCDI II - Program Partnerships - 2008
    • one proposal PNCDI II - Program Ideas - 2008
    • one proposal FP7 - Ideas - 2008
    • one project PNCDI II - Human Resources - for young researchers reintegration (Dr. Antonio Radoi)
  • V. One book regarding the progress in the field of sensors and biosensors for food quality control

CONTACT
  • Dr. Biochim. Mihaela Badea
    m_badea@yahoo.it


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